Analysis of Microplastic Abundance in Sediments of Lake Limboto
Abstract
Microplastics are a form of environmental pollution that can accumulate in aquatic sediments and impact ecosystem quality. Lake Limboto is a significant aquatic ecosystem in Gorontalo Province currently facing various environmental issues, including pollution and waste accumulation. This study aimed to analyse microplastic abundance in sediments at several locations in Lake Limboto and to determine differences in abundance based on environmental characteristics and human activities near the sampling sites. We sampled four locations: an area far from settlements, Pentadio Barat Village, an area near settlements, and an area near a waste disposal site. Sediment samples were collected approximately 5 m from the lake edge at a depth of 50 cm using a shovel. The samples were oven-dried at 110°C for 24 hours, pulverised, and then sieved using a 250-mesh sieve. A 15 g portion of sediment from each location was mixed with 50 mL of saturated NaCl solution, left to stand for 24 hours, and then filtered through Whatman No. 42 filter paper using vacuum filtration. Microplastics trapped on the filter paper were subsequently examined under a microscope. The results showed the highest microplastic abundance near the waste disposal site (333.3 particles/kg), while the lowest abundance was in Pentadio Barat Village (66.66 particles/kg). Locations near settlements had an abundance of 266.6 particles/kg, whereas locations far from settlements had 200 particles/kg.
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References
Ajay, K., Behera, D., Bhattacharya, S., Mishra, P. K., Ankit, Y., & Anoop, A. (2021). Distribution and characteristics of microplastics and phthalate esters from a freshwater lake system in Lesser Himalayas. Chemosphere, 283, 131132. https://doi.org/10.1016/j.chemosphere.2021.131132
Akbar, S. A., Afriani, S., Nuzlia, C., Nazlia, S., & Agustina, S. (2023). Microplastics in sediment of Indonesia waters : A systematic review of occurrence, monitoring and potential environmental risks. Depik, 12(3), 259-273. https://doi.org/10.13170/depik.12.3.34596
Belontz, S. L., Corcoran, P. L., de Haan-Ward, J., Helm, P. A., & Marvin, C. (2022). Factors driving the spatial distribution of microplastics in nearshore and offshore sediment of Lake Huron, North America. Marine Pollution Bulletin, 179, 113709. https://doi.org/10.1016/j.marpolbul.2022.113709
Bhardwaj, L. K., Rath, P., Yadav, P., & Gupta, U. (2024). Microplastic contamination, an emerging threat to the freshwater environment: a systematic review. Environmental Systems Research, 13(1), 8. https://doi.org/10.1186/s40068-024-00338-7
Buwono, N. R., Risjani, Y., & Soegianto, A. (2022). Spatio-temporal patterns of occurrence of microplastics in the freshwater fish Gambusia affinis from the Brantas River, Indonesia. Environmental Pollution, 311, 119958. https://doi.org/10.1016/j.envpol.2022.119958
Cordova, M. R., Ulumuddin, Y. I., Purbonegoro, T., & Shiomoto, A. (2021). Characterization of microplastics in mangrove sediment of Muara Angke Wildlife Reserve, Indonesia. Marine Pollution Bulletin, 163, 112012. https://doi.org/10.1016/j.marpolbul.2021.112012
Crutchett, T. W., & Bornt, K. R. (2024). A simple overflow density separation method that recovers >95% of dense microplastics from sediment. MethodsX, 12, 102638. https://doi.org/10.1016/j.mex.2024.102638
Cutroneo, L., Reboa, A., Geneselli, I., & Capello, M. (2021). Considerations on salts used for density separation in the extraction of microplastics from sediments. Marine Pollution Bulletin, 166, 112216. https://doi.org/10.1016/j.marpolbul.2021.112216
Dusaucy, J., Gateuille, D., Perrette, Y., & Naffrechoux, E. (2021). Microplastic pollution of worldwide lakes. Environmental Pollution, 284, 117075. https://doi.org/10.1016/j.envpol.2021.117075
Ephsy, D., & Raja, S. (2023). Characterization of microplastics and its pollution load index in freshwater Kumaraswamy Lake of Coimbatore, India. Environmental Toxicology and Pharmacology, 101, 104207. https://doi.org/10.1016/j.etap.2023.104207
Gran, A., Vidal-Barrachina, D., Casado-Coy, N., Encizo-Cuartas, D., Masó-Herrero, S., & Sanz-Lazaro, C. (2025). Comparing methods for optimising microplastic extraction in sediment through density separation. Environmental Pollution, 383, 126894. https://doi.org/10.1016/j.envpol.2025.126894
Huang, Y., Fan, J., Liu, H., & Lu, X. (2022). Vertical distribution of microplastics in the sediment profiles of the Lake Taihu, eastern China. Sustainable Environment Research, 32(1), 44. https://doi.org/10.1186/s42834-022-00154-7
Jarosz, K., Natkański, P., & Michalik, M. (2022). Microplastic Extraction from the Sediment Using Potassium Formate Water Solution (H2O/KCOOH). Minerals, 12(2), 269. https://doi.org/10.3390/min12020269
Maisto, M., Ranauda, M. A., Zuzolo, D., Tartaglia, M., Postiglione, A., Prigioniero, A., Falzarano, A., Scarano, P., Sciarrillo, R., & Guarino, C. (2022). Influence of sediment texture on HDPE microplastics recovery by density separation. Journal of Environmental Management, 317, 115363. https://doi.org/10.1016/j.jenvman.2022.115363
Monteiro, S. S., Rocha-Santos, T., Prata, J. C., Duarte, A. C., Girão, A. V., Lopes, P., Cristovão, T., & da Costa, J. P. (2022). A straightforward method for microplastic extraction from organic-rich freshwater samples. Science of The Total Environment, 815, 152941. https://doi.org/10.1016/j.scitotenv.2022.152941
Neelavannan, K., Sen, I. S., Lone, A. M., & Gopinath, K. (2022). Microplastics in the high-altitude Himalayas: Assessment of microplastic contamination in freshwater lake sediments, Northwest Himalaya (India). Chemosphere, 290, 133354. https://doi.org/10.1016/j.chemosphere.2021.133354
Range, D., Scherer, C., Stock, F., Ternes, T. A., & Hoffmann, T. O. (2023). Hydro-geomorphic perspectives on microplastic distribution in freshwater river systems: A critical review. Water Research, 245, 120567. https://doi.org/10.1016/j.watres.2023.120567
Saad, D., Ndlovu, M., Ramaremisa, G., & Tutu, H. (2022). Microplastics in freshwater environment: the first evaluation in sediment of the Vaal River, South Africa. Heliyon, 8(10), e11118. https://doi.org/10.1016/j.heliyon.2022.e11118
Sajad, S., Allam, B. K., Mushtaq, Z., & Banerjee, S. (2023). Microplastic Detection and Analysis from Water and Sediment: A Review. Macromolecular Symposia, 407(1), 2100367. https://doi.org/10.1002/masy.202100367
Viitala, M., Steinmetz, Z., Sillanpää, M., Mänttäri, M., & Sillanpää, M. (2022). Historical and current occurrence of microplastics in water and sediment of a Finnish lake affected by WWTP effluents. Environmental Pollution, 314, 120298. https://doi.org/10.1016/j.envpol.2022.120298







